phenotype microarray biolog pm1  (Biolog Inc)

Journal: Nature Communications

Article Title: Dietary carbohydrates alter immune-modulatory functionalities and DNA inversions in Bacteroides thetaiotaomicron

doi: 10.1038/s41467-025-60202-9

Figure Lengend Snippet: a Experimental design, conditioned media from carbon sources in which B. theta grew after 24 h was sterile filtered and introduced to SPF mice splenocytes. Five days later, cell population percentages were evaluated by flow cytometry analysis and IL-10 and IL-17 cytokine concentrations in the media were measured using ELISA. b , c Log2 fold change (compared to control, cells exposed to M9 minimal media and the relevant carbon source without any bacteria growing in it) of CD8 + Ki67 + PD1+ cell percentages of splenocytes exposed to conditioned media of B. theta grown on carbon sources from PM1 plate ( b ) and carbon sources from PM2A plate ( c ). d , e Log2 fold change (compared to control, cells exposed to M9 minimal media and the relevant carbon source without any bacteria growing in it) of IL-10 concentration in the media of splenocytes exposed to conditioned media of B. theta grown on carbon sources from PM1 plate ( d ) and carbon sources from PM2A plate ( e ). f , g Log2 fold change (compared to control, cells exposed to M9 minimal media and the relevant carbon source without any bacteria growing in it) of IL-17 concentration in the media of splenocytes exposed to conditioned media of B. theta grown on carbon sources from PM1 plate ( f ) and carbon sources from PM2A plate ( g ). h Heatmap representing the average log2 fold change value for each of the immune parameters examined (CD8 + Ki67 + PD1+ cell percentages, IL-10 and IL-17 concentrations). Z-score was calculated for each carbon source in each parameter. Carbon sources with |Z-score| ≥ 2 in each immune parameter were marked with #. b-g Each dot represents a biological repeat 3 ≤ n ≤ 10. b , c Kruskal–Wallis ANOVA, using Dunn’s multiple comparisons test, d , f , g Ordinary one-way ANOVA, using Tukey’s multiple comparisons test, e Brown-Forsythe and Welch ANOVA, using Dunnett’s T3 multiple comparisons test. Bars represent the mean. Error bars represent s.d. * P < 0.05 and ** P < 0.01, *** P < 0.001, **** P < 0.0001, all exact p -values are listed under “P-values” sheet in the Source Data file. Source data are provided as a Source Data file. Figure was created in BioRender. Geva-Zatorsky, N. (2025) https://BioRender.com/82criwi .

Article Snippet: To examine the suspected effects, B. theta was grown in M9 minimal media with 190 distinct carbon sources using PM1 and PM2A Biolog Phenotype MicroArraysTM plates for 24 h. Bacterial growth was assessed by both OD 600 measurements and colony-forming unit (CFU) counts (Supplementary Fig. ).

Techniques: Sterility, Flow Cytometry, Enzyme-linked Immunosorbent Assay, Control, Bacteria, Concentration Assay

Journal: Nature Communications

Article Title: Dietary carbohydrates alter immune-modulatory functionalities and DNA inversions in Bacteroides thetaiotaomicron

doi: 10.1038/s41467-025-60202-9

Figure Lengend Snippet: a , b Log2 fold change (compared to control, cells exposed to M9 minimal media and the relevant carbon source, boiled or not boiled, without any bacteria growing in it) of CD8 + Ki67 + PD1+ cell percentages of splenocytes exposed to conditioned media of B. theta grown on carbon sources from PM1 plate ( a ) and carbon sources from PM2A plate ( b ). Darker colors represent not boiled media, lighter colors represent boiled media. c , d Log2 fold change (compared to control, cells exposed to M9 minimal media and the relevant carbon source, boiled or not boiled, without any bacteria growing in it) of IL-10 concentrations in the media of splenocytes exposed to conditioned media of B. theta grown on carbon sources from PM1 plate ( c ) and carbon sources from PM2A plate ( d ). Darker colors represent not boiled media, lighter colors represent boiled media. Log2 fold change (compared to control, cells exposed to M9 minimal media and the relevant carbon source, boiled or not boiled, without any bacteria growing in it) of IL-17 concentrations in the media of splenocytes exposed to conditioned media of B. theta grown on carbon sources from PM1 plate ( e ) and carbon sources from PM2A plate ( f ). Darker colors represent not boiled media, lighter colors represent boiled media. a – f Each dot represents a biological repeat 6 ≤ n ≤ 9. Paired, tow tailed, t test for each couple of not boiled and boiled conditioned media. Bars represent the mean. Error bars represent s.d. * P < 0.05 and ** P < 0.01, *** P < 0.001, **** P < 0.0001, all exact p -values are listed under “P- values” sheet in the Source Data file. Source data are provided as a Source Data file.

Article Snippet: To examine the suspected effects, B. theta was grown in M9 minimal media with 190 distinct carbon sources using PM1 and PM2A Biolog Phenotype MicroArraysTM plates for 24 h. Bacterial growth was assessed by both OD 600 measurements and colony-forming unit (CFU) counts (Supplementary Fig. ).

Techniques: Control, Bacteria

Journal: bioRxiv

Article Title: Acquired Amphotericin B Resistance Attributed to a Mutated ERG3 in Candidozyma auris

doi: 10.1101/2025.03.30.646105

Figure Lengend Snippet: Significant Growth of Amphotericin B Susceptible Isolate Compared to Resistant Isolate Biolog Phenotypic plates PM1 and PM2a were inoculated with LNV001 and LNV002. Optical density was measured every 6-8 hours for 72 hours. Blue line is LNV001 and red line is LNV002. Standard deviation is denoted by the blurring surrounding the lines. Significance determined by a student’s T-test with the Bonferroni correction applied (α = 0.000263).

Article Snippet: The high-throughput phenotypic screen was performed utilizing Biolog PM1 and PM2a phenotypic microarray plates (Biolog, Inc., Hayward, CA) per manufacturer’s protocol for Saccharomyces cerevisiae .

Techniques: Standard Deviation